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KMID : 1024520140230061095
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Journal of the Environmental Sciences
2014 Volume.23 No. 6 p.1095 ~ p.1100
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Characteristics of Bacteria-Originated Keratinase for Feather Waste Treatment
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Go Tae-Hun
Lee Sang-Mi
Cho Kwang-Sik
Lee Ye-Ram
Park Soo-Yun
Jang Eun-Young
Jeong Seong-Yun
Son Hong-Joo
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Abstract
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Keratin wastes are generated in excess of million tons per year worldwide and biodegradation of keratin by
microorganisms possessing keratinase activity can be used as an alternative tool to prevent environmental pollution.
For practical use of keratinase, its physicochemical properties should be investigated in detail. In this study, we
investigated characteristics of keratinase produced by Xanthomonas sp. P5 which is isolated from rhizospheric soil of
soybean. The level of keratinase produced by the strain P5 increased with time and reached its maximum (10.6 U/ml)
at 3 days. The production of soluble protein had the same tendency as the production of keratinase. Optimal
temperature and pH of keratinase were 40℃-45℃ and pH 9, respectively. The enzyme showed broad temperature and
pH stabilities. Thermostability profile showed that the enzyme retained 94.6%-100% of the original activity after 1 h
treatment at 10℃-40℃. After treatment for 1 h at pH 6-10, 89.2%-100% of the activity was remained. At pH 11, 71.6%
of the original activity was retained after 1 h treatment. Although the strain P5 did not degrade human hair, it degraded
duck feather and chicken feather. These results indicate that keratinase from Xanthomonas sp. P5 could be not only
used to upgrade the nutritional value of feather hydrolysate but also useful in situ biodegradation of feather.
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KEYWORD
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Feather, Keratin, Keratinase, Xanthomonas sp.
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